Genetic dissection of the control of normal differentiation in myeloid leukemic cells

Abstract

Normal myeloid precursors and MGI+D+ myeloid leukemic cells can be induced to differentiate to mature cells by the normal protein inducer MGI [macrophage and granulocyte iducer]. The sequence of differentiation is the induction of C3 [complement] and Fc rosettes, C3 and Fc immune phagocytosis (IP), synthesis and secretion of lysozyme and formation of mature macrophages and granulocytes. Mutant clones of mouse myeloid leukemic cells were isolated with differences in the time of induction of C3 and Fc rosettes and C3 and Fc IP, in which lysozyme was induced without going through the stage of Fc or C3 IP, and differences in inducibility by MGI to mature macrophages or granulocytes. Only 1 of 5 MGI-D- clones gave rise to MGI+D+ mutants. The ability to obtain mutants from this clone was associated with its special chromosome constitution and these mutants showed a change in their ability for cap formation by concanavalin A. The steroid inducer dexamethasone can induce macrophage differentiation in MGI+D+ clones but not granulocytes. Differentiation by steroid inducer in different clones occurred with or without induction of Fc rosettes and Fc IP, and induction of C3 rosettes was not always associated with induction of C3 IP. The use of mutants that differ in their competence to be induced by MGI or steroid inducer showed that there are separate controls for the induction of C3 and Pc rosettes, C3 and Fc IP. lysozyme, macrophages and granulocytes.