Inhibitor analysis of calf thymus DNA polymerases α, δ and ϵ
- 14 March 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 341 (1), 128-130
- https://doi.org/10.1016/0014-5793(94)80254-8
Abstract
Quantitative effects of inhibitors of the replicative DNA polymerases (pol) α, δ and ϵ from calf thymus are reported under similar assay conditions. Carbonyldiphosphonate was a competitive inhibitor of pols δ and ϵ, with 4- to 6-fold selectivity compared to pol α. Aphidicolin inhibited pols α and δ with 6- to 10-fold selectivity compared to pol ϵ. The ‘butylphenyl’ nucleotides, BuPdGTP and BuAdATP, inhibited pol α with at least 1000-fold selectivity compared to pols δ and ϵ. The use of these inhibitors under similar assay conditions permits the discrimination of the three enzymesKeywords
This publication has 15 references indexed in Scilit:
- DNA polymerase ε: in search of a functionTrends in Biochemical Sciences, 1992
- Deoxyribonucleotide analogs as inhibitors and substrates of DNA polymerasesPharmacology & Therapeutics, 1990
- Revised nomenclature for eukaryotic DNA polymerasesEuropean Journal of Biochemistry, 1990
- Carbonyldiphosphonate, a selective inhibitor of mammalian DNA polymerase .delta.Biochemistry, 1989
- Calf thymus DNA polymerase δ Independent of proliferating cell nuclear antigen (PCNA)Nucleic Acids Research, 1989
- Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary proteinNature, 1987
- Cyclin/PCNA is the auxiliary protein of DNA polymerase-δNature, 1987
- Quantitative structure-activity relationships of 6-anilinouracils as inhibitors of Bacillus subtilis DNA polymerase IIIJournal of Medicinal Chemistry, 1984
- Purification of deoxyribonucleic acid polymerase .delta. from calf thymus: partial characterization of physical propertiesBiochemistry, 1980
- A new mammalian DNA polymerase with 3' to 5' exonuclease activity: DNA polymerase δBiochemistry, 1976