Flow cytometric analysis of cellular endogenous fluorescence simultaneously with emission from exogenous fluorochromes, light scatter and absorption
Open Access
- 1 July 1981
- Vol. 2 (1), 39-43
- https://doi.org/10.1002/cyto.990020109
Abstract
A cytofluorimeter capable of simultaneous 4‐parameter analysis (i.e., endogenous fluorescence (NAD(P)H), exogenous fluorescent dyes, absorption and small angle scatter) is described. Analyses have been performed on isolated rat liver cells, white blood cells, bone marrow, thymus cells and yeast under different metabolic states. This allows correlations between cell function and concentration of cell substances, size, refractive indices, etc. Kinetic measurements are also possible.Keywords
This publication has 5 references indexed in Scilit:
- Time: A New Parameter for Kinetic Measurements in Flow CytometryScience, 1980
- Studies on Metabolic Events in Localized Compartments of the Living Cell by Rapid MicrospectrofluorometryPublished by Elsevier ,1974
- [61] High-yield preparation of morphologically intact isolated parenchymal cells from rat liverMethods in Enzymology, 1974
- A comparative study of pyridine nucleotide metabolism in yeast and mammalian cells by microfluorimetry-microelectrophoresisHistochemistry and Cell Biology, 1967
- Localization and Kinetics of Reduced Pyridine Nucleotide in Living Cells by MicrofluorometryJournal of Biological Chemistry, 1959