Adsorption of Cytoplasmic and Wall-bound Saccharase of Sugar Beet Seedlings to Cell Wall

Abstract

Cytoplasmic and wall-bound saccharases were prepared from the seedlings of sugar beet, and adsorption-release characteristics of the enzymes were compared by the use of a partially purified wall Adsorption of the bound enzyme occurred below 0.25 m in NaCl concentration and was unaffected by changes in pH, while the cytoplasmic enzyme was adsorbed only in acidic media of pH 3~4. Release of re-adsorbed enzymes with NaCl depended on its concentration, and the cytoplasmic enzyme was liberated more readily than the bound type, giving their maximum values at 0.2 m and 0.8 m NaCl, respectively. Thus, the two saccharases showed merked differences in adsorption to and release from the wall. Adsorption of the cytoplasmic enzyme to the wall was reversible with changes in pH of the medium. The results suggested that the wall-bound saccharase was essentially different from the cytoplasmic enzyme and consequently not an artifact.