Sulfated glycosaminoglycans in amyloid plaques of prion diseases
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Acta Neuropathologica
- Vol. 77 (4), 337-342
- https://doi.org/10.1007/bf00687367
Abstract
Brain sections from cases of human Creutzfeldt-Jakob disease, Gerstmann-Sträussler syndrome, kuru, and hamster scrapie containing amyloid were examined for the presence of sulfated glycosaminoglycans (GAGs), the anionic component of proteoglycans, using the sulfated Alcian blue method and Alcian blue technique with 0.3 M and 0.7 M magnesium chloride. These studies suggest that sulfated glycosaminoglycans are part of the CNS amyloid plaques in each of the above human prion disorders as well as in experimental scrapie. All the amyloid plaques stained positively with Alcian blue at 0.3 M, and less so at 0.7 M magnesium chloride indicating the presence of sulfated GAGs. Therefore, the amyloid plaques of prion diseases possess similar histochemical features to those found in Alzheimer's disease.Keywords
This publication has 44 references indexed in Scilit:
- A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitorsNature, 1988
- Ultrastructural studies of glycoconjugates in brain micro-blood vessels and amyloid plaques of scrapie-infected miceActa Neuropathologica, 1988
- Prions and Neurodegenerative DiseasesNew England Journal of Medicine, 1987
- Identification of prion amyloid filaments in scrapie-infected brainCell, 1985
- Antibodies to paired helical filaments in Alzheimer's disease do not recognize normal brain proteinsNature, 1983
- Novel Proteinaceous Infectious Particles Cause ScrapieScience, 1982
- Measurement of the scrapie agent using an incubation time interval assayAnnals of Neurology, 1982
- AMYLOID PLAQUES IN THE BRAINS OF MICE INFECTED WITH SCRAPIE: MORPHOLOGICAL VARIATION AND STAINING PROPERTIESNeuropathology and Applied Neurobiology, 1975
- Comparison of Scrapie and Transmissible Mink Encephalopathy in Hamsters. II. Clinical Signs, Pathology, and PathogenesisThe Journal of Infectious Diseases, 1975
- PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPYJournal of Histochemistry & Cytochemistry, 1974