Ethidium bromide binding to core particle: comparison with native chromatin
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (10), 3231-3254
- https://doi.org/10.1093/nar/6.10.3231
Abstract
Ethidium bromide intercalation into DNA of nuclease digested erythrocyte chromatin and core particle, was followed at low ionic strength by fluorescence measurements, equilibrium dialysis using 14C labelled dye, circular dichroism and electron microscopy. High affinity binding sites in the chromatin are no more present in the core particle, i.e. when the linker is removed. In the case of core particle, a cooperative process occurs, accompanied by a partial stripping of the DNA from the core histone. Finally two populations of core particles can be detected by electron microscopy as far as their binding properties are concerned.Keywords
This publication has 33 references indexed in Scilit:
- Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: Evidence for a DNA superhelixJournal of Molecular Biology, 1978
- Relaxation of chromatin structure induced by ethidium binding: 1-micrococcal nuclease digestion of the ethidium — Chromatin complexBiochemical and Biophysical Research Communications, 1978
- Ethidium bromide (EB) binding to nucleosomal DNAExperimental Cell Research, 1977
- Relaxation of chromatin structure by ethidium bromide binding: determined by viscometry and histone dissociation studiesBiochemistry, 1976
- Binding of ethidium bromide to fractionated chromatinExperimental Cell Research, 1976
- Thermal denaturation of the DNA-ethidium complex. Redistribution of the intercalated dye during meltingBiochemistry, 1975
- Structure of deoxyribonucleoproteins. III. Spectroscopic characterization of the ethidium bromide binding sitesBiochemistry, 1974
- Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonucleaseBiochemical and Biophysical Research Communications, 1973
- Binding of ethidium bromide to avian erythrocyte chromatinBiochemical and Biophysical Research Communications, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970