Developmental genetics of a temperature-sensitive RNA polymerase II mutation in Drosophila melanogaster
- 1 September 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 187 (1), 120-125
- https://doi.org/10.1007/bf00384394
Abstract
Summary Purified RNA polymerase II (RNA nucleotidyl-transferase; EC 2.7.7.6) extracted from flies possessing lesions in the Ultrabithorax-like (Ubl) locus of Drosophila melanogaster has altered activity in vitro (Greenleaf et al. 1979, 1980; Coulter and Greenleaf 1982). This strongly suggests that the Ubl locus encodes a subunit of RNA polymerase II. Ethyl methanesulfonate was used to induce a temperature-sensitive mutation in this locus. Flies either homozygous or hemizygous for this new X−linked mutation (Ubl ts) display viability comparable to that of wild-type flies at 22° C but are lethal at 29° C. The temperature-sensitive period for Ubl ts flies is between gastrulation (6 h, 29° C) and pupation (9–10 days, 22° C). Zygotes shifted from 22° C to 29° C die at either the late embryonic or first larval instar stage while temperature shifts of second and third instar larvae result in the lethal phase occurring at the pupal stage. Most pupae shifted from 22° C to 29° C undergo metamorphosis and eclose as adults. Adults are viable if placed at 29° C; however, all females and some males become sterile if maintained at this temperature. Somatic recombination was used to induce clones homozygous for a null allele of Ubl at different stages of development. Clones of this null allele appear to be cell lethal indicating that the Ubl + gene product is required at all stages of development. The viability of Ubl ts pupae and adults at 29° C may result from only a partial reduction in activity caused by the mutation at this nonpermissive temperature.Keywords
This publication has 12 references indexed in Scilit:
- Properties of mutationally altered RNA polymerases II of Drosophila.Journal of Biological Chemistry, 1982
- An RNA polymerase II mutation in Drosophila melanogaster that mimics ultrabithoraxChromosoma, 1981
- DNA-dependent RNA polymerases from Drosophila melanogaster adults: Isolation and partial characterizationBiochemical Genetics, 1981
- Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogasterCell, 1980
- α-amanitin-resistant D. melanogaster with an altered RNA polymerase IICell, 1979
- Regulation and metamorphosis of the abdominal histoblasts ofDrosophila melanogasterWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1979
- Rates of synthesis of major classes of RNA in Drosophila embryosDevelopmental Biology, 1979
- Histological analysis of the dynamics of growth of imaginal discs and histoblast nests during the larval development ofDrosophila melanogasterWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1977
- Autoradiographic study of protein and RNA formation during early development of Drosophila eggsDevelopmental Biology, 1976
- Some parameters of mitotic recombination in Drosophila melanogasterMolecular Genetics and Genomics, 1972