A transcription terminator in the 5′ non‐coding region of the tyrosyl tRNA synthetase gene from Bacillus stearothermophilus
- 1 August 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 158 (3), 505-510
- https://doi.org/10.1111/j.1432-1033.1986.tb09783.x
Abstract
The 5'' non-coding region of the tyrosyl-tRNA synthetase gene (tyrS) of Bacillus stearothermophilus is 324 nucleotides long. It contains a premature terminator and a strong promoter: these were identified in vitro by RNA run-off experiments and in Escherichia coli by construction of specific mutants. The terminator consists of a stem and loop structure followed by the string of T residues characteristic of rho-independent termination. This is preceded by another stem and loop structure which may permit the formation of an anti-terminator. Neither the promoter nor the premature terminator appears to be regulated by the tyrosyl-tRNA synthetase in vitro or in E. coli.This publication has 38 references indexed in Scilit:
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