The assay and reaction kinetics of leucine aminopeptidase from swine kidney
- 1 March 1964
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 90 (3), 509-512
- https://doi.org/10.1042/bj0900509
Abstract
A sensitive precise pH-stat assay for peptidases is described. The Michael is parameters for a number of substrates of leucine aminopeptidase have been measured. Leucine aminopeptidase hydrolyses L-leucine benzyl ester at one-quarter the rate of L-leucine amide. Leucine aminopeptidase is inhibited by glycerol and butanol, and the dissociation constants of the alcohol complexes of the enzyme and of the enzyme-substrate complex have been computed.This publication has 8 references indexed in Scilit:
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