PROPERTIES OF A TEMPERATURE-SENSITIVE, RADIATION-SENSITIVE MUTANT OF Escherichia coli , II. DNA REPLICATION
Open Access
- 1 December 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (4), 1195-1202
- https://doi.org/10.1073/pnas.64.4.1195
Abstract
We have described a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. In vitro assays have demonstrated that DNA ligase activity is markedly reduced in this strain relative to that of the parent strain. Okazaki fragments of newly replicated DNA accumulate in this mutant strain upon growth at the restrictive temperature. These results imply that the same enzyme, DNA ligase, functions both in normal semiconservative replication and in nonconservative repair replication of DNA.Keywords
This publication has 8 references indexed in Scilit:
- Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli.Proceedings of the National Academy of Sciences, 1968
- Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.Proceedings of the National Academy of Sciences, 1968
- Intermediates in T4 DNA Replication in a T4 Ligase Deficient StrainCold Spring Harbor Symposia on Quantitative Biology, 1968
- Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.Proceedings of the National Academy of Sciences, 1967
- Formation of covalent circles of lambda DNA by E. coli extracts.Proceedings of the National Academy of Sciences, 1967
- Evidence for repair-replication of ultraviolet damaged DNA in bacteriaJournal of Molecular Biology, 1964
- ISOLATION OF THE GROWING POINT IN THE BACTERIAL CHROMOSOMEProceedings of the National Academy of Sciences, 1964
- Involvement of Synthesis of RNA in Thymineless DeathNature, 1963