A Selective Radioimmunoassay for the Determination of Pirenzepine in Plasma and Urine
- 1 June 1987
- journal article
- research article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 9 (2), 236-242
- https://doi.org/10.1097/00007691-198706000-00019
Abstract
A radioimmunoassay procedure for the determination of pirenzepine in either plasma or urine was demonstrated to be both sensitive and specific as well as highly reproducible. The assay could detect levels as low as 1.25 ng/ml. The sensitivity was sufficient to allow the analysis of biological samples from both pharmacokinetic and clinical studies. The two metabolites of pirenzepine, LS 75 and LS 822, did not cross-react with the antiserum. The assay was not affected by a change in anticoagulant or by the presence of several over-the-counter or prescription drugs, even at very high levels. Samples could be frozen and stored for at least a year without affecting the analysis. Repeat analysis could be performed on samples that had been re-frozen. Several thousand plasma and urine samples, including plasma samples from severely renally impaired patients, have been analyzed for pirenzepine by the RIA with no interferences having been detected.Keywords
This publication has 4 references indexed in Scilit:
- Automated monoclonal radioimmunoassays for pirenzepine, a selective muscarinic receptor antagonist, in plasma and urineJournal of Immunological Methods, 1986
- Effect of food and antacids on the oral absorption of pirenzepine in manInternational Journal of Pharmaceutics, 1986
- Determination of Pirenzepine in Dosage Forms and in Biological FluidsJournal of Liquid Chromatography, 1984
- Muscarinic receptor subtypes: M1 and M2 biochemical and functional characterizationLife Sciences, 1982