Sequencing technology does not eliminate biological variability
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Open Access
- 11 July 2011
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 29 (7), 572-573
- https://doi.org/10.1038/nbt.1910
Abstract
RNA sequencing has generated much excitement for the advantages offered over microarrays. This excitement has led to a barrage of publications discounting the importance of biological variability; as microarray publications did in the 1990s. By comparing microarray and sequencing data, we demonstrate that expression measurements exhibit biological variability across individuals irrespective of measurement technology. Our analysis suggests RNA-sequencing experiments designed to estimate biological variability are more likely to produce reproducible results.Keywords
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