Formation of Neutralizing Antibody in Monkeys Injected with Poliomyelitis Virus and Adjuvants.

Abstract

The formation of neutralizing antibodies in rhesus monkeys is enhanced when the antigen, spinal cord containing poliomyelitis virus, is incorporated into a water-in-oil emulsion. The addition of a small amt. of killed Mycobacterium butyricum to the water-in-oil emulsion further augments antibody formation. Some of the monkeys, however, develop fatal allergic encephalitis. By repeating the injns. of antigen without these adjuvants, antibody titers can be produced which are equal to those found after the injn. of antigen in water-in-oil emulsion, but not as high as in monkeys receiving the combination of antigen, paraffin oil and killed M. butyricum.