Rapid analysis of DNA strand breaks in soft tissues
- 1 January 1985
- journal article
- research article
- Published by Wiley in Environmental Mutagenesis
- Vol. 7 (6), 871-880
- https://doi.org/10.1002/em.2860070608
Abstract
A technique was developed to measure rapidly DNA strand breaks in soft tissues. This method measured the rate of alkaline unwinding of DNA, which was proportional to strand breakage. Alkaline unwinding of DNA was done by treating tissue homogenates with NaOH. Single‐stranded DNA was removed by extraction with aqueous phenol. DNA unwinding was quantitated by measuring the remaining double‐stranded DNA. Using the described technique, a dose‐effect relationship was observed between N‐nitrosodimethylamine (NDMA) and alkaline unwinding of mouse liver DNA.Keywords
This publication has 18 references indexed in Scilit:
- Quantitation of chemically induced DNA strand breaks in human cells via an alkaline unwinding assayAnalytical Biochemistry, 1985
- DNA breaks induced by micromolar concentrations of dimethylnitrosamine in liver primary cell cultures from untreated and phenobarbital treated ratsToxicology, 1983
- A circular channel crucible oscillating viscometerJournal of Molecular Biology, 1981
- A modified fluorometric method for the determination of microgram quantities of DNA from cell or tissue culturesAnalytical Biochemistry, 1976
- Studies on the metabolism of dimethylnitrosamine in the rat. I. Effect of dose, route of administration and sexFood and Cosmetics Toxicology, 1975
- Radiation Induced Strand Breakage in DNA from Mammalian CellsInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1973
- Repair of a DNA Complex from X-irradiated Chinese Hamster CellsInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1972
- Sedimentation of DNA Released from Chinese Hamster CellsBiophysical Journal, 1971
- Reconstruction in vivo of Irradiated Escherichia coli Deoxyribonucleic Acid; the Rejoining of Broken PiecesNature, 1966