A Reference System in Crossed Radioimmunoelectrophoresis

Abstract

The reference system described here takes into account the variable quality of the 125I-labeled anti-Ig[immunoglobulin]E and permits a direct comparison of the results from 1 experiment to another. Simultaneously with the preparation of the CRIE (crossed radioimmunoelectrophoresis) slides, a standard series of small paper discs are prepared containing varying, defined amounts of 125I-labeled anti-IgE. These paper discs are laid out for autoradiography together with the CRIE slides. After short periods of exposure, only paper discs with large amounts of isotope are visible on the X-ray films. Longer exposure times permit paper discs with smaller amounts of isotope to be visible. Each precipitate is assigned to a CRIE class depending on the number of paper discs visible when the precipitate is detected for the 1st time.