IgG Subclasses in Human γ‐Globulin Preparations for Intravenous Use and Their Reactivity with Staphylococcus Protein A1
- 5 March 1980
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 38 (3), 147-155
- https://doi.org/10.1111/j.1423-0410.1980.tb02342.x
Abstract
In 2 of 4 non-enzymatically treated .gamma.-globulin preparations C Immunoglobulin [Ig] Schura, Immunoglobulin SRK, the distribution of IgG subclasses was close to that of normal human serum. In 2 other preparations (sulfonated and .beta.-propiolactone-treated) IgG3 was not detectable by appropriate antiserum. The IgG residual portion of plasmin-treated .gamma.-globulin was enriched in IgG2, while IgG3 was absent. In affinity chromatography on protein A Sepharose, IgG3 in the unbound and IgG1, IgG2 and IgG4 in the bound fractions were found in Immunoglobulins Schura and SRK. In the sulfonated preparation no IgG was found in the unbound fraction, while IgG1, IgG2 and IgG4 were eluted from the bound fraction. In .beta.-propiolactone-treated .gamma.-globulin IgG1, IgG2 and IgG4 were present in both fractions. The testing of reactivity of IgG subclasses with Staphylococcus protein A can supply important information about the state of the Fc part in Ig preparations.This publication has 13 references indexed in Scilit:
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