Detection of Chloramphenicol Acetyl Transferase Activity in Transfected Cells: A Rapid and Sensitive HPLC-based Method
- 1 December 1985
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 4 (6), 469-475
- https://doi.org/10.1089/dna.1985.4.469
Abstract
An assay based on high-performance liquid chromotography (HPLC) has been developed for determining the expression of the acetyl coenzyme A: chloramphenicol 3-O-acetyltransferase gene in transfected cells. The assay is based on the separation of extracted, nonradioactive chloramphenicol and its acetylated derivatives by reverse-phase HPLC, using UV absorbance for detection. In addition to eliminating the need for costly [14C]chloramphenicol, this assay is as sensitive and much faster than the commonly used method involving thin-layer chromatography.This publication has 7 references indexed in Scilit:
- Immunoglobulin Heavy-Chain Enhancer Requires One or More Tissue-Specific FactorsScience, 1985
- Use of gene transfer to study expression of steroid-responsive genesMolecular and Cellular Endocrinology, 1984
- Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genesNature, 1983
- Multiple Point Mutations Affecting the Simian Virus 40 EnhancerScience, 1983
- Chloramphenicol Acetyltransferase: Enzymology and Molecular BiologyCritical Reviews in Biochemistry, 1983
- Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.Molecular and Cellular Biology, 1982
- SV40-transformed simian cells support the replication of early SV40 mutantsCell, 1981