Balance between Metabolite Accumulation and Transport in Relation to Photosynthesis by Isolated Spinach Chloroplasts
Open Access
- 1 April 1980
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 65 (4), 574-577
- https://doi.org/10.1104/pp.65.4.574
Abstract
The relationship between the rate of orthophosphate (Pi) transport into the stroma and the rate of CO2 fixation by intact chloroplasts was investigated. High Pi concentrations in the medium lead to a depletion of stromal metabolites, due to excessive Pi transport into the stroma, resulting in the inhibition of CO2 fixation. This inhibitory effect of Pi is released by inhibitors of Pi transport, such as pyrophosphate, citrate or pyridoxal-5-phosphate. The latter compound appeared to be specially valuable in inhibiting Pi transport without affecting stromal reactions.Keywords
This publication has 7 references indexed in Scilit:
- Specific transport of inorganic phosphate, 3-phosphoglycerate and triosephosphates across the inner membrane of the envelope in spinach chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1978
- p-Chloromercuriphenyl sulphonic acid as a specific inhibitor of the phosphate transporter in isolated chloroplastsFEBS Letters, 1977
- The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitroBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
- Site of Action of Inhibitors of Carbon Dioxide Assimilation by Whole Lettuce ChloroplastsPlant Physiology, 1975
- The reduction of 3-phosphoglycerate by reconstituted chloroplasts and by chloroplast extractsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1974
- Photosynthesis by isolated chloroplasts. Inhibition by dl-glyceraldehyde of carbon dioxide assimilationBiochemical Journal, 1972
- Photosynthesis by isolated chloroplasts I. Diffusion of labeled photosynthetic intermediates between isolated chloroplasts and suspending mediumBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1968