Polynucleotide-Protein Interactions in the Translation System. Detection of Contacts between Some Ribosomal Split Proteins and 16-S RNA in 30-S Subunits of Escherichia coli Ribosomes
- 1 September 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 90 (1), 83-88
- https://doi.org/10.1111/j.1432-1033.1978.tb12577.x
Abstract
Direct contacts between 16-S RNA and split proteins S2, S3, S5, S14 and S21 inside the 30-S subunit of E. coli ribosomes were evidenced by the formation of UV-induced (.lambda. = 254 nm) RNA-protein cross-links. Subunits (30-S) were reassembled from core particles and a mixture of split proteins containing in each case a single 125I-labeled protein. All the proteins tested are cross-linked as a result of a single-hit process; proteins S3 and S21 were cross-linked at the highest rate.This publication has 21 references indexed in Scilit:
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