INHIBITION OF RIBONUCLEIC ACID SYNTHESIS IN NEWCASTLE DISEASE VIRUS-INFECTED CELLS BY PUROMYCIN AND 6-AZAURIDINE
- 1 December 1964
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 88 (6), 1550-+
- https://doi.org/10.1128/jb.88.6.1550-1555.1964
Abstract
Puromycin and 6-azauridine were used to inhibit protein and ribonucleic acid (RNA) synthesis during Newcastle disease virus replication. Viral RNA, measured by the incorporation of uridine-2-C14, increased rapidly 6 to 7 hr after infection and reached a peak at 12 hr. Viral RNA synthesis was inhibited by puromycin added at the time of infection or at any time up to 4 hr after infection, but not therafter. 6-Azauridine (3 mg/ml) inhibited over 90% of the viral RNA synthesis and inhibited protein synthesis to a small extent. Infected cells synthesized more viral RNA than did untreated controls when exposed to azauridine alone for 5 hr, followed by puromycin alone for 9 hr. It was concluded that immediately after virus infection, virus-specific proteins, which are necessary for viral RNA synthesis, were synthesized. Virus-specific protein synthesis may occur even when viral RNA synthesis is greatly inhibited. After virus-specific protein synthesis has taken place, viral RNA synthesis may take place when protein synthesis is almost totally inhibited.Keywords
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