Some Practical Aspects of Measurements of Dissolved Free Amino Acids in Natural Waters and Within Microalgae by the Use of HPLC
- 1 June 1988
- journal article
- research article
- Published by Taylor & Francis in Chemistry and Ecology
- Vol. 3 (4), 269-293
- https://doi.org/10.1080/02757548808070848
Abstract
A detailed method for the analysis of dissolved free amino acids (DFAA) and ammonium by reverse phase high performance chromatography of fluorescent derivatives using commercial columns and guard columns is described in detail for the newcomer. The method is flexible enough to allow for minor differences between different HPLC pump and gradient systems. For DFAA, the method is sensitive and reproducible down to high femptomole/low picomole levels, suitable for analysis of DFAA in natural waters down to the low nanomolar range. Aspects of reproducibility, sensitivity, sample preparation, cell extraction and sample storage are considered.Keywords
This publication has 29 references indexed in Scilit:
- Nitrogen metabolism of marine planktonic diatoms; excretion, assimilation and cellular pools of free amino acids in seven species with different cell sizeJournal of Experimental Marine Biology and Ecology, 1986
- Nitrogen sources for the growth of marine microalgae: role of dissolved free amino acidsMarine Ecology Progress Series, 1986
- Biological considerations in the measurement of dissolved free amino acids in seawater and implications for chemical and microbiological studiesMarine Ecology Progress Series, 1985
- Release of aminoacids and inorganic nutrients by heterotrophic marine micro-flagellatesMarine Ecology Progress Series, 1985
- Amino Acids in Sea WaterChemistry and Ecology, 1984
- Utilization of amino acids by planktonic marine bacteria: Importance of clean technique and low substrate additions1,2Limnology and Oceanography, 1984
- Utilization of dissolved amino acids in seawater by marine bacteriaMarine Biology, 1982
- A High Performance Liquid Chromatographic Determination of Free Amino Acids in Natural Waters in the Picomolar (M X 10−12) Range Suitable for Shipboard UseChemistry and Ecology, 1982
- The relationship between inorganic and organic nutrients in sea waterJournal of the Marine Biological Association of the United Kingdom, 1979
- A note on the losses of monosaccharides, amino sugars, and amino acids from extracts during concentration proceduresAnalytical Biochemistry, 1978