Production And Evaluation Of Antisera For Serological Type Determination Of Group‐B Streptococci by Double Diffusion In Agarose Gel

Abstract

A double diffusion technique for serological typing of Group-B streptococci is presented. The method is valuable for type determination on a large scale and for evaluation of the specificity of type antisera [from rabbits, cows and goats]. HCl-extracted antigens are used. The preparation of type antisera is described and the reactions of some selected sera are given. The advantages of the method are good economy of antisera and easy recognition of weak and/or unspecific precipitates. The method is especially useful in epidemiological investigations.