Demonstration of Several Components in Highly Purified Human Growth Hormone by Immunodiffusion on Cellulose Acetate

Abstract

At least 5–6 components have been demonstrated in highly purified human growth hormone (HGH) preparations (Li or Raben) by permitting the HGH to react with its antiserum, employing a modified technique of immunodiffusion on cellulose acetate. Tests were performed with varying ratios of antigen (HGH) to antiserum. The components (precipitation lines) were usually more numerous and better defined with relative excesses of the antiserum. Levels of HGH (Li) as low as 3 f*g still revealed the presence of 3 components. The same antiserum gave no detectable reactions when tested against varying amounts of ovine prolactin, likewise a protein hormone with a molecular weight similar to that of HGH. Control tests with normal rabbit serum were also negative.