Mutations affecting catabolite repression of the L-arabinose regulon in Escherichia coli B/r

Abstract

Expression of the L-arabinose regulon in E. coli B/r requires, among other things, c[cyclic]AMP and the cAMP receptor protein (CRP). Mutants deficient in adenyl cyclase (cya-), the enzyme which synthesizes cAMP, or CRP (crp-) are unable to utilize a variety of carbohydrates, including L-arabinose. Ara+ revertants of a cya- crp- strain were isolated on 0.2% minimal L-arabinose plates, conditions which require the entire ara regulon to be activated in the absence of cAMP and CRP. Evidence from genetic and physiological studies is consistent with placing these mutations in the araC regulatory gene. Deletion mapping with 1 mutant localized the site within either araO or araC, and complementation tests indicated the mutants acted trans to confer the ability to utilize L-arabinose in a cya- crp- genetic background. Since genetic analysis supports the conclusion that the mutant sites are in the araC regulatory gene, the mutants were designated araCi, indicating a mutation in the regulatory gene affecting the cAMP-CRP requirement. Physiological analysis of 1 mutant, araCi1, illustrates the trans-acting nature of the mutation. In a cya- crp- genetic background, araCi1 promoted synthesis of isomerase, a product of the araBAD operon, and permease, a product of the araE operon. Isomerase and permease levels in araCi1 cya+ crp+ were hyperinducible and the sensitivity of each to cAMP was altered. Two models are presented that show the possible mutational lesion in the araCi strains.