Simple Method for Visualization of the Islets in Fixed But Otherwise Intact Pancreas

Abstract
A simple, inexpensive, reproducible method is described for prominently displaying the islets of Langerhans. The method consists of sequential arterial perfusion of the organ of the sacrificed animal [rabbits, rats or mice] with saline, formalin, hematoxylin and water, followed by clearing in methyl salicylate. The procedure should be useful whenever islet tissue needs to be quantitatively distinguished from nonislet tissue and fixation is allowable.