Relationship between histone H1 poly(adenosine diphosphate-ribosylation) and histone H1 phosphorylation using anti-poly(adenosine diphosphate-ribose) antibody
- 1 May 1983
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (10), 2384-2389
- https://doi.org/10.1021/bi00279a013
Abstract
The chromatin associated enzyme poly(ADP-Rib[ribose]) polymerase catalyzes the posttranslational modification of histones. Antibody to poly(ADP-Rib) was coupled to Sepharose, and the resultant immunoadsorbent was used to fractionate, specifically, histone H1 subpopulations undergoing this nuclear protein modification. When this method of separation was used, it was additionally observed that poly(ADP-ribosylated) H1 species were highly accessible to in vitro phosphorylation by nuclear protein kinase. Phosphorylated H1 molecules were retained by the anti-poly(ADP-Rib)-Sepharose column due to the presence of endogenous poly(ADP-Rib) components. Degradation of the latter moieties on phosphorylated H1 reversed their adsorption to the column.This publication has 23 references indexed in Scilit:
- Characterization of Poly(ADP-ribose)-Histone H1 Complex Formation in Purified Polynucleosomes and ChromatinEuropean Journal of Biochemistry, 2005
- The Participation of Poly(ADP-Ribosyl)ated Histone H1 in Oligonucleosomal CondensationEuropean Journal of Biochemistry, 2005
- Phosphorylation states of different histone 1 subtypes and their relationship to chromatin functions during the HeLa S-3 cell cycleBiochemistry, 1981
- Comparison of the structures of human histones 1A and 1B and their intramolecular phosphorylation sites during the HeLa S-3 cell cycleBiochemistry, 1981
- Relationship between nicotinamide adenine dinucleotide concentration and in vitro synthesis of poly(adenosine diphosphate ribose) on purified nucleosomesBiochemistry, 1980
- Histone H2A subfractions and their phosphorylation in cultured cellsExperimental Cell Research, 1980
- Properties of the Complex between Histone H1 and Poly(ADP‐ribose) Synthesised in HeLa Cell NucleiEuropean Journal of Biochemistry, 1977
- Some effects of chlorambucil on nuclear protein phosphorylation in the yoshida ascites sarcomaChemico-Biological Interactions, 1977
- Poly(adenosine diphosphate-ribose) polymerase: the distribution of a chromosome-associated enzyme within the chromatin substructureBiochemistry, 1977
- Changes in poly(adenosine diphosphate-ribose) and poly(adenosine diphosphate-ribose) polymerase in synchronous HeLa cellsBiochemistry, 1976