RADIOIMMUNOASSAY OF FOLLICLE-STIMULATING (FSH) AND LUTEINIZING (LH) HORMONES BY CHROMATOELECTROPHORESIS

Abstract

Procedure for the radioimmunoassay of FSH and LH using chromatoelectrophoresis for the separation of antibody-bound and free labeled hormone is described. Diurnal variation in FSH and LH levels of normal men and women in follicular and luteal phases of the cycle is reported. The diurnal variation was not abolished in three women taking contraceptive medication and in two women in postmenopause. The diurnal variation of FSH and LH in plasma is compared with that of cortisol, dehydroepiandrosterone and testosterone. It is suggested that diurnal variation of FSH and LH may be independent of a normally functioning pituitary-gonadal axis; however, steroidal feed-back may have a regulatory effect on the magnitude of the diurnal variation. The plasma levels of FSH and LH in normal subjects during normal menstrual cycles as well as in patients with endocrinopathies obtained in our laboratory by radioimmunoassay are compared with those obtained by both radioimmunoassay and bioassay by other investigators.