Use of Cellulose Acetate Electrophoresis as an Alternative to Starch Gel Electrophoresis for Detecting Root-Knot Nematode Resistance in Tomato

Abstract

Detection of the acid phosphatase-1 (Aps-1) isozyme from tomato genotypes susceptible, homozygous resistant, and heterozygous resistant to root-knot nematode [Meloidogyne incognita, M. arenaria, M. javanica] was compared by cellulose acetate and starch gel electrophoresis. In contrast to starch gel electrophoresis, cellulose acetate electrophoresis made discrete separations of Aps-1 bands and gave better resolution. In all tests, cellulose acetate electrophoresis was quicker and more economical than starch gel electrophoresis in assaying for the Aps-1 isozyme.