Polycythemia vera. The in vitro response of normal and abnormal stem cell lines to erythropoietin.
Open Access
- 1 April 1978
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 61 (4), 1044-1047
- https://doi.org/10.1172/jci109003
Abstract
Bone marrow cells from two glucose-6-phosphate dehydrogenase (G-6-PD) heterozygotes with polycythemia vera were cultured to determine whether progenitors which wre not of the polycythemia vera clone were present, and, if present, which cell lines contributed to the increase in erythroid colonies observed in response to added erythropoietin (ESF). To accomplish this, the G-6-PD isoenzyme activity of individual erythroid colonies was determined. All of the erythroid colonies analyzed in cultures without added ESF, contained the G-6-PD isoenzyme type characteristic of the abnormal clone. With higher ESF concentrations in the culture, however, there was an increase in the colonies that were not of the polycythemia vera clone. Analysis of the ratio of the various types of colonies indicated that normal and polycythemia vera cells are capable of responding to ESF in vitro. In selected patients, this technique permits analysis of the ratios of normal to abnormal cells during the course of the disease, in response to therapy and during late complications, such as myelofibrosis or leukemic transformation.This publication has 16 references indexed in Scilit:
- Chronic myelocytic leukemia: Clonal origin in a stem cell common to the granulocyte, erythrocyte, platelet and monocyte/macrophageAmerican Journal Of Medicine, 1977
- POLYCYTHEMIA-VERA - HORMONAL MODULATION OF ERYTHROPOIESIS INVITRO1977
- Human erythroid colony formation in vitro: Evidence for clonal originJournal of Cellular Physiology, 1976
- Polycythemia Vera: Stem-Cell and Probable Clonal Origin of the DiseaseNew England Journal of Medicine, 1976
- TIME-LAPSE CHARACTERIZATION OF ERYTHROCYTIC COLONY-FORMING CELLS IN PLASMA CULTURES1976
- Bone-Marrow Responses in Polycythemia VeraNew England Journal of Medicine, 1974
- Primordial cell pool size and lineage relationships of five human cell types*Annals of Human Genetics, 1973
- Cellulose acetate electrophoresis of human glucose-6-phosphate dehydrogenase.1969
- The Erythropoietin/Hematocrit Relationship in Normal and Polycythemic Man: Implications of Marrow RegulationBlood, 1968
- Studies on the response of hypoxia and relative hyperoxia in two polycythemia vera patients.1967