Induction of cycloheximide resistance in Candida maltosa by modifying the ribosomes.

Abstract

When Candida maltosa IAM12247 was grown in the presence of cycloheximide, the growth was repressed for a distinct period of time depending on the concentration of cycloheximide, then the growth recovered ("adapted"). The cell-free translation experiments using polyuridylic acid as mRNA indicated that the protein-synthesizing activity was altered in the cells adapted to resist cycloheximide. A reconstituted cell-free system was constructed consisting of ribosomes and soluble fraction (S-100), and it was concluded that ribosomal modification was induced during the cultivation of the cells in the presence of cycloheximide.