Japanese B Encephalitis Virus in Tissue Culture.
- 1 March 1957
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 94 (3), 556-560
- https://doi.org/10.3181/00379727-94-23011
Abstract
Two strains of Japanese B encephalitis (JBE) virus were propagated serially in tissue cultures of chick-embryo fibroblasts, monkey kidney epithelium, HeLa cells, and Detroit-6 cells. Cyto-pathogenicity which was consistent and reproducible was observed only with the Detroit-6 cell line. Maximal virus growth (105 to 106 mouse LD 50/ml of tissue culture fluid) was seen 2 days before cytopathogenicity became pronounced. The cytopathogenicity was never 100% complete and cell sheets were observed to recover and virus proliferation continue when nutrient fluids were regularly replaced. Cytopathogenicity was readily neutralized by JBE immune serum. A similar cytopathogenic effect was observed in Detroit-6 cells infected with the Egypt 101 strain of West Nile virus.Keywords
This publication has 3 references indexed in Scilit:
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- THE SPECIFIC AMINO ACID REQUIREMENTS OF A HUMAN CARCINOMA CELL (STRAIN HELA) IN TISSUE CULTUREThe Journal of Experimental Medicine, 1955
- THE VIRAL RANGE INVITRO OF A MALIGNANT HUMAN EPITHELIAL CELL (STRAIN HELA, GEY) .2. STUDIES WITH ENCEPHALITIS VIRUSES OF THE EASTERN, WESTERN, WEST NILE, ST-LOUIS, AND JAPANESE B-TYPES1954