Structure and sequence of the mitochondrial 20S rRNA and tRNA tyr gene of Paramecium primaurelia
Open Access
- 11 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (23), 6391-6406
- https://doi.org/10.1093/nar/9.23.6391
Abstract
The sequence and structure of the large (20s) mitochondrial (mt) rRNA gene and flanking regions from Paramecium primaurelia have been determined. The gene contains two regions of strong homology with other large mt rRNAs: one 44-base region near the 5′ end and a 321-base region near the 3′ end. Another region of strong homology to both ends of E. coli 23s RNA exists at loci consistent with these regions. The Paramecium gene appears to be 2204 bases in length and contains slightly more homology to E. coli rRNA than its mammalian or fungal counterparts. The gene, located about 1200 bp from the replicative terminal end of the linear mt DNA, is transcribed in the same polarity as replication. Previous R-looping studies detected no large introns within the gene. Here we describe sequences resembling degenerate rRNAs, one of which could represent a small intron. A tRNA tyr gene was found on the same DNA strand, 127 bp downstream from the large rRNA presumptive 3′ end. The tRNA is flanked on both sides by short DNA regions of −90% A + T content.Keywords
This publication has 39 references indexed in Scilit:
- Sequencing of the 16S–23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genesCell, 1981
- Mitochondrial tRNA gene clusters in Aspergillus nidulans: Organization and nucleotide sequenceCell, 1981
- The 3′ terminus of the large ribosomal subunit (“17S”) RNA from hamster mitochondria is ragged and oligoadenylatedCell, 1981
- Sequence homologies between Escherichia coli and chloroplast ribosomal DNA as seen by heteroduplex analysisJournal of Molecular Biology, 1980
- Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the ω and rib-1 lociCell, 1980
- Splice point sequence and transcripts of the intervening sequence in the mitochondrial 21S ribosomal RNA gene of yeastCell, 1980
- Organization of tRNA and rRNA genes in N. crassa mitochondria: Intervening sequence in the large rRNA gene and strand distribution of the RNA genesCell, 1979
- Mitochondrial DNA replication in Paramecium aurelia. Cross-linking of the initiation endJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Structure and replication of mitochondrial DNA from Paramecium aureliaJournal of Molecular Biology, 1975