Identification of a key domain in annexin and 14‐3‐3 proteins that stimulate calcium‐dependent exocytosis in permeabilized adrenal chromaffin cells
- 12 April 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 320 (3), 207-210
- https://doi.org/10.1016/0014-5793(93)80587-k
Abstract
Calcium-dependent secretion in digitonin-permeabilized adrenal chromaffin cells is stimulated by exogenous annexin II and 14-3-3 proteins. These proteins share a conserved domain that has been suggested to be involved in specific protein-protein interactions. We examined whether this domain was involved in secretion by using a synthetic peptide (P16) of sequence KGDYQKALLYLCGGDD corresponding to the C-terminus of annexin II. P16, but not truncated peptides, prevented the stimulation of secretion by 14-3-3 proteins and produced a partial inhibition of control secretion. These data suggest that the shared annexin/14-3-3 domain is important in the mechanisms controlling Ca2+-dependent secretion and may play a key role in protein-protein interactions during exocytosis.Keywords
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