RNA Splice Site Selection: Evidence for a 5′ → 3′ Scanning Model
- 24 June 1983
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 220 (4604), 1351-1355
- https://doi.org/10.1126/science.6304877
Abstract
Human G gamma-globin genes containing tandem duplications of the donor (5') or acceptor (3') RNA splice sites of the second intervening sequence were constructed in order to ascertain the directionality of RNA splice site selection. These genes were introduced into cultured monkey cells, and their transcripts were analyzed. Transcripts of these duplication variants were spliced only at the proximal copy of the duplicated splice sites. These data are consistent with a 5' leads to 3' model of splice site selection.Keywords
This publication has 76 references indexed in Scilit:
- Expression of a β-globin gene is enhanced by remote SV40 DNA sequencesCell, 1981
- The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinantsNucleic Acids Research, 1981
- The 20,000-dalton variant of human growth hormone: Location of the amino acid deletionsBiochemical and Biophysical Research Communications, 1980
- Mutants of SV40 with an altered small t protein are reduced in their ability to transform cellsCell, 1978
- Two adenovirus mRNAs have a common 5′ terminal leader sequence encoded at least 10 kb upstream from their main coding regionsCell, 1977
- A precursor of globin messenger RNAJournal of Molecular Biology, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolutionJournal of Molecular Biology, 1975
- The lac represser-operator interactionJournal of Molecular Biology, 1970
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967