Study of Pah's Separation and Phase-Solute Interaction by Micellar Liquid Chromatography

Abstract

The large k' values obtained in micellar liquid chromatography of Brij-35 on C-18 and C-8 columns clearly decrease in the presence of methanol or propanol as organic modifiers, propanol being superior to methanol although resolution is better with the latter. A linear relationship between k,' not log k', and the number of carbons is obtained. The distribution coefficient of the solutes between the stationary and aqueous phases increases, as do the micelle-solute association constants, with the number of aromatic rings for naphthalene, phenanthrene, chrysene and dibenzo(ah)anthracene. Sensitivity is increased with respect to that obtained in the classic isocratic RPLC methanol/water method. In best conditions nine PAH's are resolved to the baseline.