INVIVO DOSIMETRY OF 4-AMINOBIPHENYL IN RATS VIA A CYSTEINE ADDUCT IN HEMOGLOBIN
- 1 January 1984
- journal article
- research article
- Vol. 44 (10), 4254-4259
Abstract
The feasibility of monitoring doses of 4-aminobiphenyl (ABP) via adduction to Hb was investigated. Rats dosed with ABP (from 0.5-5 mg/kg) formed a stable covalent Hb:ABP adduct. Approximately 5% of a single dose was bound as Hb:ABP; chronic dosing led to an accumulation of the adduct to a level 30 times greater than that found after a single dose. Facile in vitro hydrolysis of the adduct regenerated ABP, allowing detection at the sub-ng level. Human Hb was also readily adducted, using N-hydroxy-ABP in vitro. The predominant site of adduction appeared to be the cysteine residue in Hb. The use of such adducts as dosimeters for arylamine exposures in humans is discussed.This publication has 6 references indexed in Scilit:
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