Discrimination Between Sporothrix schenckii and Ceratocystis stenoceras Rhamnomannans by Proton and Carbon-13 Magnetic Resonance Spectroscopy
- 1 April 1974
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 9 (4), 674-680
- https://doi.org/10.1128/iai.9.4.674-680.1974
Abstract
The proton and 13C magnetic resonance (CMR) spectra of 10 Sporothrix schenckii and three Ceratocystis stenoceras rhamnomannans were studied. On the basis of their CMR spectra, the rhamnomannans from S. schenckii strains could be classified into two major types, I and II, readily distinguishable from the polysaccharides formed at the same temperature (25 C) by C. stenoceras. Rhamnomannans from S. schenckii synthesized at 37 C gave CMR spectra (type III) differing from those of polysaccharides of types I and II and from those of C. stenoceras polysaccharides. The contention that C. stenoceras might be the perfect form of S. schenckii is not supported by the present data. A presumptive pathogenic mutant of C. stenoceras synthesized a type I rhamnomannan characteristic of S. schenckii, clearly differing from the rhamnomannans from C. stenoceras synthesized in the same conditions. Nuclear magnetic resonance spectroscopy revealed differences in the structures of the polysaccharides from these species not previously recognized by methylation analysis.Keywords
This publication has 4 references indexed in Scilit:
- [Adaptation of Ceratocystis to a parasitic life in animals--aquisition of a pathogenicity comparable to Sporothrix schenckii].1971
- A comparison of some Ceratocystis species with sporothrix schenckiiMycopathologia, 1970
- Systematics of the generaSaccharomyces, Schizosaccharomyces, Endomycopsis, Kluyveromyces, Schwanniomyces andBrettanomyces: Proton magnetic resonance spectra of the mannans and mannose-containing polysaccharides as an aid in classificationAntonie van Leeuwenhoek, 1969
- Mannose-containing polysaccharides of the apiculate yeasts Nadsonia, Hanseniaspora, Kloeckera, and Sacharomycodes, and their use as an aid in classification.1968