Forms of vitamin B6 in human milk

Abstract

A previously developed high performance chromatographic method has been modified slightly and used to determine the forms of vitamin B₆ in human milk. The chromatographic traces are free of compounds that would interfere with the two principal forms found, pyridoxal and pyridoxal phosphate. The method is fast and reliable; it yields recoveries of from 90 to 106% for the vitamers and agrees with results obtained on the same samples with the standard microbiological assay.