Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent
- 15 June 2004
- journal article
- research article
- Published by Optica Publishing Group in Optics Letters
- Vol. 29 (12), 1396-1398
- https://doi.org/10.1364/ol.29.001396
Abstract
We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and of phyA.
Keywords
This publication has 9 references indexed in Scilit:
- Engineered microsphere contrast agents for optical coherence tomographyOptics Letters, 2003
- Molecular contrast in optical coherence tomography by use of a pump–probe techniqueOptics Letters, 2003
- The Phytochromes, a Family of Red/Far-red Absorbing PhotoreceptorsJournal of Biological Chemistry, 2001
- Optical coherence tomography (OCT): a reviewIEEE Journal of Selected Topics in Quantum Electronics, 1999
- In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomographyOptics Letters, 1997
- Two-dimensional birefringence imaging in biological tissue by polarization-sensitive optical coherence tomographyOptics Letters, 1997
- The green fluorescent protein as a marker to visualize plant mitochondria in vivoThe Plant Journal, 1997
- Optical coherence tomographyJournal of Biomedical Optics, 1996
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990