The Use of Cytochalasin B to Distinguish Myoblasts from Fibroblasts in Cultures of Developing Chick Striated Muscle
- 1 September 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9), 3621-3625
- https://doi.org/10.1073/pnas.71.9.3621
Abstract
Cytochalasin B (5 mug/ml) elicits a differential effect on myoblasts and fibroblasts in culture. After 1 day in culture in the presence of the drug, two types of cells were observed, round cells and cells with elongated arms, designated "arborized" cells. Both cell types were examined in the electron microscope. The round cells contained aggregates of thin and thick filaments as well as a few intact sarcomeres. Within the arms of the arborized cells were bundles of intermediate sized filaments (100 A in diameter). The round cells could be shaken off the culture dish, washed free of cytochalasin B, and recultured to form myotubes. The remaining arborized cells lost their stellate shape when the drug was removed. The progeny of these cells gave rise to normal fibroblasts. Cytochalasin B, thus could be used to identify and isolate myoblasts prior to their fusion into developing muscle. It is suggested that this differential effect of the drug can be used to prepare pure cultures of fusible muscle cells uncontaminated by fibroblasts.Keywords
This publication has 22 references indexed in Scilit:
- Cytochalasin B and the sialic acids of Ehrlich ascites cellsExperimental Cell Research, 1974
- Cytochalasin‐B: Effects on cytokinesis, glycogen and 3H‐D‐glucose incorporationJournal of Anatomy, 1972
- Cytochalasin B: Inhibition of D-2-Deoxyglucose Transport into Leukocytes and FibroblastsScience, 1972
- Muscle development in vitro A new conditioned medium effect on colony differentiationExperimental Cell Research, 1971
- Cytochalasin B: Microfilaments and "Contractile" ProcessesScience, 1971
- Microfilaments in Cellular and Developmental ProcessesScience, 1971
- In vitro myogenesis of promuscle cells from the regenerating tail of the lizard,Anolis carolinensis,Journal of Morphology, 1968
- MITOSIS AND INTERMEDIATE-SIZED FILAMENTS IN DEVELOPING SKELETAL MUSCLEThe Journal of cell biology, 1968
- Effects of Cytochalasins on Mammalian CellsNature, 1967
- Clonal Analysis of MyogenesisScience, 1963