Performance of an Integrated Biosensor Composed of a Mediated and an Oxygen-Based Glucose Sensor Under Unknown Oxygen Tension

Abstract
An integrated micro-glucose sensor which was apparently unaffected by the concentration of dissolved oxygen was developed. This sensor consisted of both a mediated glucose sensor (MGS) and an O2-based glucose sensor (OGS) using glucose oxidase. The responses of both MGS and OGS were affected by concentration of dissolved oxygen. However, the responses of MGS were corrected on the basis of those of OGS. Then the precise glucose concentration was obtained from the following equations under several oxygen concentrations. Y+2. 1×10−1 Z+1.9×10−3Z2−6.4×10−5Z3+5. 7×10−9Z4= 6.5×10−1G+2.5×10−3G2−2. 0×10−5G3+4.7×10−8G4−3.7×10−11G 5 X = 104/(103V+G) where X, Y, Z, G and V are glucose concentration (mg/dl), response of MGS (nA), response of OGS (nA), injection volume of 10 g/dl glucose solution (μl) and initial volume (ml), respectively.