USE OF GEL FILTRATION FOR INSULIN IMMUNOASSAY

Abstract

Gel filtration was tested for radio-immunoassay of plasma insulin, as a mean for separating antibody-bound insulin from the residual free insulin. Using Sephadex G 100 columns, the curves of the 2 fractions, bound and free, were found to be dissociated within an error of 2.0% for eluate collection. The results of the basic experiments showed that this procedure is suitable for immunoassay of insulin as well as for that of other peptide hormones of relatively low molecular weight.