Effects of vasoactive substances and cAMP related compounds on adrenomedullin production in cultured vascular smooth muscle cells
Open Access
- 7 August 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 369 (2-3), 311-314
- https://doi.org/10.1016/0014-5793(95)00769-6
Abstract
To elucidate the regulation mechanism of adrenomedullin (AM) production in blood vessels, we examined the effects of 30 substances on AM production in cultured rat vascular smooth muscle cells (VSMCs). Forskolin and 8‐bromo‐cAMP suppressed production and gene transcription of AM. Since VSMC expresses AM receptors coupled with adenylate cyclase, AM production may be regulated by intracellular cAMP concentration. Thrombin, vasoactive intestinal polypeptide and interferon‐gg also inhibited AM production, while angiotensin II, endothelin‐1, bradykinin, substance P, adrenaline, phorbol ester and fetal calf serum stimulated AM production in VSMC. These results suggest that AM production is regulated by a variety of substances, indicating complex systems regulating AM production.Keywords
This publication has 22 references indexed in Scilit:
- Interleukin-1, Tumor Necrosis Factor and Lipopolysaccharide Additively Stimulate Production of Adrenomedullin in Vascular Smooth Muscle CellsBiochemical and Biophysical Research Communications, 1995
- Distribution and characterization of immunoreactive rat adrenomedullin in tissue and plasmaFEBS Letters, 1994
- Genomic Structure of Human Adrenomedullin GeneBiochemical and Biophysical Research Communications, 1994
- Adrenomedullin Stimulates Cyclic AMP Formation in Rat Vascular Smooth Muscle CellsBiochemical and Biophysical Research Communications, 1994
- Promoter Analysis of Human Inducible Nitric Oxide Synthase Gene Associated with Cardiovascular HomeostasisBiochemical and Biophysical Research Communications, 1994
- Specific receptors for adrenomedullin in cultured rat vascular smooth muscle cellsFEBS Letters, 1994
- Molecular Cloning and Biological Activities of Rat Adrenomedullin, a Hypotensive PeptideBiochemical and Biophysical Research Communications, 1993
- Adrenomedullin: A Novel Hypotensive Peptide Isolated from Human PheochromocytomaBiochemical and Biophysical Research Communications, 1993
- Cloning of Inducible Nitric Oxide Synthase in Rat Vascular Smooth Muscle CellsBiochemical and Biophysical Research Communications, 1993
- Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cellsBiochemical and Biophysical Research Communications, 1985