The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain
- 1 April 1970
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 117 (2), 341-346
- https://doi.org/10.1042/bj1170341
Abstract
Stem bromelain that had been irreversibly inhibited with 1,3-dibromo[2-14C]-acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and α-chymotrypsin three radioactive peptides were isolated chromatographically. The amino acid sequences around the cross-linked cysteine and histidine residues were determined and showed a high degree of homology with those around the active-site cysteine and histidine residues of papain and ficin.Keywords
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