Characterization of DNA-protein crosslinks formed by treatment of L1210 cells and nuclei with bis(2-chloroethyl)methylamine (nitrogen mustard)
- 19 September 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (19), 3954-3958
- https://doi.org/10.1021/bi00612a012
Abstract
Proteins cross-linked to DNA after nitrogen mustard (HN2) treatment of [mouse leukemia L-1210] cells or isolated nuclei were purified in CsCl gradients. The protein-DNA cross-links could be cleaved by incubation in dilute acid and could be stabilized by alkali pretreatment. These results indicated that proteins cross-linked to DNA by HN2 were bound to alkylated purines. Analysis of the DNA-bound protein on NaDodSO4[sodium dodecyl sulfate]-polyacrylamide gels showed that primarily large nonhistone proteins were cross-linked to DNA in cells treated with HN2. Very little, if any, histone was cross-linked to the DNA. Comparison of DNA bound proteins from HN2-treated cells and HN2-treated nuclei showed that in general the same proteins were linked to DNA in both cases, but some qualitative and quantitative differences exist.This publication has 6 references indexed in Scilit:
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