Isopycnic Ultracentrifugation of Immune Complexes
- 1 January 1985
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 21 (1), 93-98
- https://doi.org/10.1111/j.1365-3083.1985.tb01407.x
Abstract
Isopycnic ultracentrifugation is frequently applied for preparative isolation of macromolecules. Using bovine serum albumin (BSA)-anti-BSA antibody complexes as a model system, isopycnic banding of complexes was observed in CsCl, Nycodenz, and sucrose gradients. In CsCl gradients, free antigen or antibody could not be separated from the immune complexes. Variations in antigen to antibody ratio from equivalence and in the amount of complement present during complex formation resulted in zone broadening and banding at slightly lower densities in Nycodenz. This was not observed in sucrose. Serum immune complexes were isolated from patients with rheumatoid arthritis or ankylosing spondylitis. Banding of in vivo-formed immune complexes was observed more frequently in sucrose than in Nycodenz.Keywords
This publication has 9 references indexed in Scilit:
- Preparative Isolation of Immune Complexes from Serum by Sucrose Gradient UltracentrifugationScandinavian Journal of Immunology, 1984
- A technique for the identification of glycoprotein antigens in immune complexes and its application to the detection of a common glycomprotein in sera of patients with burkitt's lymphoma and nasopharyngeal carcinomaJournal of Immunological Methods, 1981
- Biochemical Aspects of Immune Complex Formation and Immune Complex DiseasesAllergy, 1980
- Analysis of the components of immune complexesMolecular Immunology, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A new complement function: solubilization of antigen-antibody aggregates.Proceedings of the National Academy of Sciences, 1975
- The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassayBiochemical Journal, 1973
- Molecular composition and sedimentation characteristics of soluble antigen-antibody complexesBiochemistry, 1972
- An unusual pressure dependence for a reversibly associating protein system; sedimentation studies on myosin.Proceedings of the National Academy of Sciences, 1967