Enzymic dissection of embryonic cell adhesive mechanisms: III. *Immunological identification of a component of the calcium-dependent adhesive system of embryonic chick neural retina cells

Abstract

Immunological probes and cell-surface biochemical analysis are combined to identify a cellsurface glycoprotein, which appears to be involved in the formation of calcium-dependent adhesions among embryonic chick neural retina cells. Comparison of radioiodinated cells shows that adhesive-competent cells have at their surface two glycoproteins not present on nonadhesive cells. These two components (with M, 130 × 10³, pl 4·8 and M_r 70 × 10³, pl 4×8) are immunoprecipitable with adhesion-blocking antisera. A fraction derived from retina-conditioned medium, containing one major immunoprecipitable component, neutralizes the inhibitory activity of aggregation-inhibiting Fab’ fragments and competes in immunoprecipitation for the two glycoprotein components. The evidence suggests that one or both of these components is involved in the formation of calcium-dependent adhesions among embryonic chick neural retina cells.