Further Investigation of a Temperature-sensitive Strain of Tobacco Mosaic Virus: Its Behaviour in Tomato Leaf Epidermis

Abstract

A temperature-sensitive (ts) strain of tobacco mosaic virus (TMV), LS1, was further investigated with regard to its behavior at permissive (22.degree. C) and nonpermissive (32.degree. C) temperatures at the primary infection sites in tomato leaf epidermis. Tomato leaflets were inoculated with LS1 or a wild-type strain, L, cultured at 22 or 32.degree. C and the epidermis isolated; virus spread was followed by staining with fluorescent antibody after enzymic digestion to the upper cell wall. Profiles of epidermis stained with fluorescent antibody indicated that LS1 multiplies in primary-infected cells but that it cannot move to the neighboring cells at the nonpermissive temperature.