The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG‐ and CCAAT sequences in single stranded oligonucleotides
- 31 January 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 338 (2), 157-160
- https://doi.org/10.1016/0014-5793(94)80355-2
Abstract
We have characterized the nucleic acid binding properties of the major cold shock protein of Bacillus subtilis, CspB. CspB is a member of the cold shock domain (CSD) family, which is widespread among pro- and eukaryotes and shares the nucleic acid binding domain CSD. The CSD domain is highly conserved and binds with strong affinity to the Y-box motif, a cis-element that contains the CTG AA sequence. In a series of gel retardation experiments using oligonucleotides, which contain the Y-box motif and altered sequences, we show the preferential binding of CspB to single-stranded DNA that contains the ATTGG as well as the complementary CCAAT Y-box core sequence. In contrast CspB exhibits lower affinity to altered Y-box core sequences. Dependent on the length of the oligonucleotide and the degree of sequence deviation from the Y-box core sequence 3- to over 10-fold overexcess of CspB was needed for complete retardation.
Keywords
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