Microvascular filling pattern in rat liver sinusoids during vagal stimulation.

Abstract

The terminal microcirculation in the transilluminated ventral margin of the rat liver was observed and recorded by a video-microscope system. The volumetric flow rate in a liver sinusoid was calculated from the observed diameter of the sinusoid and the intra-sinusoid erythrocyte flow velocity. The topographic distribution of liver sinusoids within an arbitrary boundary of a microscopic field of terminal liver microcirculation was observed and the total inflow and outflow in the field were determined. Both vagus nerves at the lower end of the esophagus were stimulated at supramaximal voltage. Vagal stimulation dilated the caliber of liver sinusoids and paradoxically diminished the erythrocyte flow velocity in each individual liver sinusoid, but the total volumetric flows in a microscopic field remained unchanged. Vagal stimulation also increased the number of liver sinusoids in a microscopic field by opening previously closed liver sinusoids. This recruitment contributed 2/3 of the total increase of the sinusoidal capacity while the other 1/3 was the result of distension of existing liver sinusoids.